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KMID : 1129720190360010052
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Korean Journal of Acupuncture
2019 Volume.36 No. 1 p.52 ~ p.62
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Effects of Zingiberis Rhizoma Pharmacopuncture Injected at GB30 and ST36 on Neuropathic Pain in Rats
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Hwang Min-Seop
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Abstract
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Objectives : The objective of this study was to investigate the effects of Zingiberis Rhizoma Pharmacopuncture(ZP) at GB30 and ST36 in neuropathic pain induced SD rats by the block of Transient Receptor Potential Vanilloid 1(TRPV1).
Methods : Neuropathic pain in rats was induced by tibial and common peroneal nerve transection of right leg. The rat subjects were divided into 6 groups : normal(Nor, n=5), control(Con, n=5), neuropathic pain plus 2 mg/kg ZP injection at GB30 and ST36(ZP-A, n=5), 10 mg/kg ZP(ZP-B, n=5), 20 mg/kg ZP(ZP-C, n=5) and 0.45 mg/kg Tramadol(Tra, n＝5). Three days after the surgery, injections were administered once a day for 17 days. Withdrawal response of neuropathic rats’ legs were measured by stimulating the paw of Right leg with von frey filament, acetone and radient heat on day 3, 7, 11, 15, 19 after surgery. After all treatments were completed, c-Fos in the midbrain central gray and TRPV1 & TRPA1 of DRG(L5) were analyzed.
Results : Groups ZP-B and ZP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia, thermal hyperalgesia and cold allodynia compared to the control group(p＜0.05, p＜0.01, p＜0.001). Groups ZP-B and ZP-C showed a meaningful decrease in the expression of c-fos and TRPV1 protein level compared to the control group(p＜0.05, p＜0.01, p＜0.001).
Conclusions : These results suggest that Zingiberis Rhizoma Pharmacopuncture at GB30 and ST36 could decrease mechanical & cold allodynia and thermal hyperalgesia by block the TRPV1 on the model of neuropathic pain.
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KEYWORD
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neuropathic pain, Zingiberis Rhizoma, pharmacopuncture, TRPV1
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